Mobility of the maize suppressor-mutator element in transgenic tobacco cells.
نویسندگان
چکیده
منابع مشابه
Mobility of the maize suppressor-mutator element in transgenic tobacco cells.
Maize Suppressor-mutator (Spm) transposable elements have been introduced into tobacco cells and a visual assay for Spm activity has been developed using a bacterial beta-glucuronidase gene. The Spm element is mobile in tobacco and can trans-activate excision of a transposition-defective Spm (dSpm) element either from a different site on the same transforming Ti plasmid or from a second plasmid...
متن کاملTnpA trans-activates methylated maize Suppressor-mutator transposable elements in transgenic tobacco.
The maize Suppressor-mutator (Spm) transposable element is subject to epigenetic inactivation in transgenic tobacco, as it is in maize. Spm inactivation in tobacco is correlated with increased methylation of sequences near the element's transcription start site. To determine whether element-encoded gene products can promote the reactivation of an inactive element, we investigated the effects of...
متن کاملNucleotide sequence of the maize Mutator element, Mu8.
Molecular analysis of a mutant wary allele, wx-mum5 (Don that of Mul (67%), but significantly higher than the G/C content Robertson, unpublished), revealed the presence of a previously of the entire maize genome, estimated to be 49.5% G/C (2). unidentified maize Mutator element (Mu8) within the tenth exon of the waxy gene. The element is 1410 base pairs (bp) long and is flanked by a nine bp dir...
متن کاملIdentification of an Active New Mutator Transposable Element in Maize
Robertson's Mutator (Mu) system has been used in large scale mutagenesis in maize, exploiting its high mutation frequency, controllability, preferential insertion in genes, and independence of donor location. Eight Mutator elements have been fully characterized (Mu1, Mu2 /Mu1.7, Mu3, Mu4, Mu5, Mu6/7, Mu8, MuDR), and three are defined by TIR (Mu10, Mu11 and Mu12). The genome sequencing revealed ...
متن کاملRNA splicing permits expression of a maize gene with a defective Suppressor-mutator transposable element insertion in an exon.
The bz-m13CS9 allele of the bronze-1 gene in maize contains a 902-base-pair defective Suppressor-mutator (dSpm) transposable element in the second exon. Nevertheless, 40-50% of the enzymatic activity conditioned by a nonmutant allele at the bronze-1 locus is routinely recovered in crude extracts prepared from plants carrying bz-m13CS9 in the absence of an autonomous Suppressor-mutator element. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1989
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.86.7.2219